Immunology Consensus Methods

For Information about methods used for specific protocols, please contact the protocol immunologist.

Consensus Method Title	WordPerfect (w51 or wpd) or Word 97 (doc) Format	*PDF Format**
Advanced Flow Method - 10/04/04 Pediatric Advanced Flow Rationale Advanced Flow Panel Template	Flow Method Flow Rationale Flow Template	Flow Method Flow Rationale Flow Template
Antibody ELISA Tetanus ELISA - 11-02-99 Hep A (Future)	tetanus.doc	tetanus.pdf
Apoptosis Flow Cytmetric Enumeration of Apoptotic Cells Using the Tunel Assay (05/15/99)	pedsapo.doc	pedsapo.pdf
CD 38 Quantitation Method - 01/15/98 CD 38 Quantitation using the Quantibrite Assay - 04/01/99	imcd38.wpd imcd38met.doc	imcd38.pdf imcd38met.pdf
Pediatric Immunology PBMC Cryopreservation Method - 08/25/04	PBMCProcessingandcryo.jul04.doc	PBMCProcessingandcryo.jul04.pdf
CTL Method Pediatrics Bulk Method (08/31/99)	ctlbulkv2-1.doc	ctlbulkv2-1.pdf
Inducible Cytokine Method Induction of TNF alpha and IFN-Beta 11/15/96 Antigen-Specific Inducible Cytokine Method 11/99 Inducible Cytokines in Whole Blood 12/99 CD4 Antigen-Specific Cytokine Assays 10/27/99	iminducy.w51 cytokine.doc cyto-protocol.doc cd4antigen.doc	iminducy.pdf cytokine.pdf cyto-protocol.pdf cd4antigen.pdf
LPA Methods Adult and Pediatric LPA - 02/10/00 Pediatric LPA Whole Blood - 09-23-96 Pediatric LPA - Limiting Dilution Method (09/01/99)	imlpaadu.w51 imlpaped.w51 lpa-ld(rcf).doc	imlpaadu.pdf imlpaped.pdf lpa-ld(rcf).pdf
NK Method - 08/31/99	pedsnk-v1.doc	pedsnk-v1.pdf
Phagocyte Assays	phagocyt.wpd	phagocyt.pdf
Plasma Cytokine and Soluble Marker Methods Beta2 Microglobulin - 03-09-98 IMX Beta2 Microglobulin - 09-23-96 Interferon gamma - 03-09-98 Neopterin - 09-23-96 Soluble IL-2 receptor - 09-23-96 TNF alpha - 09-23-96 Human IL-2 - 08-21-98 IL-1 Beta - 08-21-98 Human IL-12 - 08-21-98 Human Rantes - 09-03-98 Human IL-4 - 03-19-99	imb2micr.wpd imb2mimx.w51 imifngam.wpd imneopte.w51 imil2r.w51 imtnf.w51 actgil2b.wpd il1b.wpd il12.wpd rante.wpd il-4.doc	imb2micr.pdf imb2mimx.pdf imifngam.pdf imneopte.pdf imil2r.pdf imtnf.pdf actgil2b.pdf il1b.pdf il12.pdf rante.pdf il-4.pdf
TCR-V Beta Repertoire PCR - 10-26-99 Flow Cytometry - 11-29-99	TCRfinal.doc flowcytometry.doc	TCRfinal.pdf flowcytometry.pdf